Soluble sugar is a key quality trait of soybean seeds. We developed rapid and economic extraction and quantification methods for seed soluble sugars using an ultra-high performance liquid chromatography system with a refractive index detector. We evaluated the soluble sugar compositions of 1164 soybean accessions collected from diverse ecoregions and grown in multiple locations and years. Total soluble sugar (TSS) content was influenced by accession type, year of cultivation, and ecoregion. The mean con-tents of fructose, glucose, sucrose, raffinose, stachyose and TSS were 3.31, 5.21, 55.60, 6.60, 35.47, and 106.19 mg g?1, respectively. The highest mean TSS content (108.71 mg g?1) was observed in accessions from Northern Region of China. Cultivars contained higher contents of sucrose, raffinose, and TSS, whereas landraces had a higher content of stachyose. Fourteen accessions with mean TSS contents>130 mg g?1 were identified as elite soybean resources. TSS was correlated with sucrose, raffinose, sta-chyose, protein, oil and total tocopherol. The main soluble sugar components were correlated with lati-tude and longitude, indicating that the geographical origin of the accessions affected their seed soluble sugar compositions. The developed methods and elite identified accessions can be used in the food and feed industry and in soybean breeding programs aimed at improving soybean seed nutrition.

Jie Qi;Shengrui Zhang;Muhammad Azam;Abdulwahab S.Shaibu;Ahmed M.Abdelghany;Yue Feng;Yuanyuan Huai;Huoyi Feng;Yitian Liu;Caiyou Ma;Berhane S.Gebregziabher;Suprio Ghosh;Jing Li;Deyue Yu;Bin Li;Lijuan Qiu;Junming Sun

The National Engineering Research Center of Crop Molecular Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Department of Agronomy,Bayero University,Kano 700001,Nigeria;Crop Science Department,Faculty of Agriculture,Damanhour University,Damanhour 22516,Egypt;National Key Laboratory of Crop Genetics and Germplasm Enhancement,National Center for Soybean Improvement,Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;MARA Key Laboratory of Soybean Biology(Beijing),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Germplasm and Biotechnology(MARA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

作物学报（英文版）

[1]Jie Qi;Shengrui Zhang;Muhammad Azam;Abdulwahab S.Shaibu;Ahmed M.Abdelghany;Yue Feng;Yuanyuan Huai;Huoyi Feng;Yitian Liu;Caiyou Ma;Berhane S.Gebregziabher;Suprio Ghosh;Jing Li;Deyue Yu;Bin Li;Lijuan Qiu;Junming Sun-.Profiling seed soluble sugar compositions in 1164 Chinese soybean accessions from major growing ecoregions)[J].作物学报（英文版）,2022(06):1825-1831

ecoregion,stachyose,chyose

Profiling,soluble,compositions,Chinese,soybean,accessions,from,major,growing,ecoregions,Soluble,is,key,quality,trait,seeds,We,developed,rapid,economic,extraction,quantification,methods,sugars,using,ultra,performance,liquid,chromatography,system,refractive,detector,evaluated,collected,diverse,grown,multiple,locations,years,Total,TSS,was,influenced,by,type,cultivation,mean,fructose,glucose,sucrose,raffinose,were,respectively,highest,observed,Northern,Region,China,Cultivars,contained,higher,contents,whereas,landraces,had,Fourteen,identified,elite,resources,correlated,protein,oil,total,tocopherol,main,components,lati,longitude,indicating,that,geographical,origin,affected,their,can,used,food,feed,industry,breeding,programs,aimed,improving,nutrition

0.490384