Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses. In rice, the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion (SL). Previous studies have identified an sl mutation (a null mutation of SL) in several rice varieties and confirmed its increase of resistance and cell death. However, a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is lacking. We performed a multi-omics analysis to clarify the funda-mental mechanisms at the protein, gene transcript, and metabolite levels. We found that cell death and Magnaporthe oryzae (M. oryzae) infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA) in multiple regula-tory layers. We characterized the dynamic changes of several key hormone levels during disease progres-sion and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease resistance. SL-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to M. oryzae. Our studies shed light on cell death and facilitate further mechanistic dissec-tion of programmed cell death in rice.

Dagang Tian;Yan Lin;Shengping Li;Yiyang Cao;Gang Li;Xinrui Guo;Ziqiang Chen;Zaijie Chen;Feng Wang;Zonghua Wang

Fujian Key Laboratory of Genetic Engineering for Agriculture,Biotechnology Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,Fujian,China;State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests,Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Plant Immunity Center,Fujian Agriculture and Forestry University,Fuzhou 350003,Fujian,China

作物学报（英文版）

[1]Dagang Tian;Yan Lin;Shengping Li;Yiyang Cao;Gang Li;Xinrui Guo;Ziqiang Chen;Zaijie Chen;Feng Wang;Zonghua Wang-.Rice sl-MH-1 mutant induces cell death and confers blast resistance via the synergistic roles of signaling systems)[J].作物学报（英文版）,2022(06):1755-1766

Sekiguchi

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