Drought stress impairs crop growth and productivity. Stress-associated proteins (SAPs), a class of zinc finger proteins containing the A20/AN1 domain, function in various stress responses in plants. However, little is known about the function of SAPs in drought-stress responses in soybean, an oil and protein crop. We report that a GmSAP5 protein confers drought tolerance by increasing sensitivity to abscisic acid (ABA) and reducing stomatal aperture. Overexpression and RNA interference of GmSAP5 in soybean hairy roots resulted in elevated resistance and sensitivity to drought stress, respectively. ABA and proline contents increased in GmSAP5-overexpressing plants under water-deficit conditions. Lower water loss rates and higher relative water contents were observed in GmSAP5-overexpressing plants, resulting in increased drought-stress resistance. A yeast one-hybrid assay and luciferase transient transcriptional activity assay showed that GmAREB3, an AREB/ABF transcription factor, could bind to the promoter of GmSAP5 and activate its expression. These results suggest that GmSAP5 acts downstream of GmAREB3 and improves drought-stress resistance by mediating ABA signaling.

Zehao Hou;Xiangzhan Zhang;Yimiao Tang;Taifei Yu;Lei Zheng;Jun Chen;Yongbin Zhou;Yongwei Liu;Ming Chen;Zhao-Shi Xu;Youzhi Ma

Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Key Laboratory of Biology and Genetic Improvement of Triticeae Crops,Ministry of Agriculture,Beijing 100081,China;Beijing Engineering Research Center for Hybrid Wheat,The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051,Hebei,China

作物学报（英文版）

[1]Zehao Hou;Xiangzhan Zhang;Yimiao Tang;Taifei Yu;Lei Zheng;Jun Chen;Yongbin Zhou;Yongwei Liu;Ming Chen;Zhao-Shi Xu;Youzhi Ma-.GmSAP5, a soybean A20/AN1 domain-containing stress-associated protein gene activated by GmAREB3, increases drought stress resistance in soybean by mediating ABA signaling)[J].作物学报（英文版）,2022(06):1601-1610

GmSAP5,GmAREB3,AREB

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