典型文献
A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield
文献摘要：
Grain size is one of the most important factors affecting rice grain quality and yield,and attracts great attention from molecular biologists and breeders.In this study,we engineered a CRISPR/Cas9 system tar-geting the miR396 recognition site of the rice GS2 gene,which encodes growth-regulating factor 4(OsGRF4)and regulates multiple agronomic traits including grain size,grain quality,nitrogen use effi-ciency,abiotic stress response,and seed shattering.In contrast to most previous genome editing efforts in which indel mutations were chosen to obtain null mutants,a mutant named GS2E carrying an in-frame 6-bp deletion and 1-bp substitution within the miR396-targeted sequence was identified.GS2E plants showed increased expression of GS2 in consistent with impaired repression by miR396.As expected,the gain-of-function GS2E mutant exhibited multiple beneficial traits including increased grain size and yield and bigger grain length/width ratio.Thousand grain weight and grain yield per plant of GS2E plants were increased by 23.5％and 10.4％,respectively.These improved traits were passed to hybrids in a semi-dominant way,suggesting that the new GS2E allele has great potential in rice improvement.Taken together,we report new GS2 germplasm and describe a novel gene-editing strategy that can be widely employed to improve grain size and yield in rice.This trait-improvement strategy could be applied to other genes containing miRNA target sites,in particular the conserved miR396-GRF/GIF module that gov-erns plant growth,development and environmental response.
文献关键词：
中图分类号：
作者姓名：
Wenshu Wang;Weipeng Wang;Yanlin Pan;Chao Tan;Hongjing Li;Ya Chen;Xingdan Liu;Jing Wei;Nian Xu;Yu Han;Han Gu;Rongjian Ye;Qi Ding;Chonglie Ma
作者机构：
Life Science and Technology Center,China National Seed Group Co.,Ltd.,Wuhan 430000,Hubei,China;State Key Laboratory of Crop Breeding Technology Innovation and Integration,China National Seed Group Co.,Ltd.,Wuhan 430000,Hubei,China
文献出处：
引用格式：
[1]Wenshu Wang;Weipeng Wang;Yanlin Pan;Chao Tan;Hongjing Li;Ya Chen;Xingdan Liu;Jing Wei;Nian Xu;Yu Han;Han Gu;Rongjian Ye;Qi Ding;Chonglie Ma-.A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield)[J].作物学报（英文版）,2022(04):1207-1212
A类：
OsGS2,GRF4,OsGRF4,GS2E,erns
B类：
new,gain,function,allele,generated,by,CRISPR,Cas9,genome,editing,increases,rice,grain,size,yield,Grain,one,most,important,factors,affecting,quality,attracts,great,attention,from,molecular,biologists,breeders,In,this,study,engineered,system,geting,miR396,recognition,which,encodes,growth,regulating,regulates,multiple,agronomic,traits,including,nitrogen,use,effi,ciency,abiotic,stress,response,seed,shattering,contrast,previous,efforts,indel,mutations,were,chosen,obtain,null,mutants,named,carrying,frame,bp,deletion,substitution,within,targeted,sequence,was,identified,plants,showed,increased,expression,consistent,impaired,repression,expected,exhibited,beneficial,bigger,length,width,ratio,Thousand,weight,per,respectively,These,improved,passed,hybrids,semi,dominant,way,suggesting,that,has,potential,improvement,Taken,together,report,germplasm,describe,novel,strategy,can,widely,employed,This,could,applied,other,genes,containing,miRNA,sites,particular,conserved,GIF,module,gov,development,environmental
AB值：
0.536843
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