Rye(Secale cereale genome RR),a close relative of common wheat,possesses valuable resistance genes for wheat improvement.Due to the co-evolution of pathogen virulence and host resistance,some resistance genes derived from rye have lost effectiveness.Development and identification of new,effective resis-tance genes from rye is thus required.In the current study,wheat-rye line WR56 was produced through distant hybridization,embryo rescue culture,chromosome doubling and backcrossing.WR56 was then proved to be a wheat-rye 2RL ditelosomic addition line using GISH(genomic in situ hybridization),mc-FISH(multicolor fluorescence in situ hybridization),ND-FISH(non-denaturing FISH),mc-GISH(mul-ticolor GISH)and rye chromosome arm-specific marker analysis.WR56 exhibited a high level of adult plant resistance to powdery mildew caused by Blumeria graminis f.sp.tritici(Bgt).This resistance was car-ried by the added 2RL telosomes and presumed to be different from Pm7 which is also located on chro-mosome arm 2RL but confers resistance at the seedling and adult stages.WR56 will be a promising bridging parent for transfer of the resistance to a more stable wheat breeding line.A newly developed 2RL-specific KASP(kompetitive allele specific PCR)marker should expedite that work.

Diaoguo An;Guohao Han;Jing Wang;Hanwen Yan;Yilin Zhou;Lijun Cao;Yuli Jin;Xiaotian Zhang

Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050022,Hebei,China;The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China;The State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China

作物学报（英文版）

[1]Diaoguo An;Guohao Han;Jing Wang;Hanwen Yan;Yilin Zhou;Lijun Cao;Yuli Jin;Xiaotian Zhang-.Cytological and genetic analyses of a wheat-rye 2RL ditelosomic addition line with adult plant resistance to powdery mildew)[J].作物学报（英文版）,2022(03):911-916

2RL,ditelosomic,Rye,WR56,backcrossing,ticolor,telosomes,Pm7

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