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典型文献
Development of high-oil maize haploid inducer with a novel phenotyping strategy
文献摘要:
Doubled haploid(DH)technology is important in modern maize breeding.Haploid inducers determine the efficiency of both haploid induction and identification.It has taken decades to improve the efficiency,haploid induction rate(HIR),from the~2%of the ancestor haploid inducer,stock6,to the~10%of modern haploid inducers.Improvement of kernel oil content(KOC)would further enhance haploid identification efficiency.Using molecular marker-assisted selection,in combine with the number of haploids per ear as phenotypic criterion,we developed a new high-oil haploid inducer line,CHOI4,with a mean HIR of 15.8%and mean KOC of 11%.High KOC of CHOI4 can achieve a mean accuracy greater than 90%in identification of haploids of different backgrounds,with reduced false discovery rates and false negative rates in com-parison with the previous high-oil haploid inducer line,CHOI3.Comparison of phenotypic selection strategies suggested that the number of haploids per ear can be used as a phenotyping criterion during haploid inducer line development.CHOI4 could further increase the efficiency of large-scale DH breeding programs with lower cost.
文献关键词:
作者姓名:
Chenxu Liu;Jinlong Li;Ming Chen;Wei Li;Yu Zhong;Xin Dong;Xiaowei Xu;Chen Chen;Xiaolong Tian;Shaojiang Chen
作者机构:
College of Agronomy and Biotechnology,Beijing Key Laboratory of Crop Genetic Improvement,Laboratory of Crop Heterosis and Utilization,Ministry of Education,National Maize Improvement Center of China,China Agricultural University,Beijing 100193,China;Chongqing Academy of Agricultural Sciences,Chongqing 401329,China
引用格式:
[1]Chenxu Liu;Jinlong Li;Ming Chen;Wei Li;Yu Zhong;Xin Dong;Xiaowei Xu;Chen Chen;Xiaolong Tian;Shaojiang Chen-.Development of high-oil maize haploid inducer with a novel phenotyping strategy)[J].作物学报(英文版),2022(02):524-531
A类:
stock6,CHOI4,CHOI3
B类:
Development,high,oil,maize,novel,phenotyping,strategy,Doubled,DH,technology,important,modern,breeding,Haploid,inducers,determine,efficiency,both,induction,identification,It,has,taken,decades,improve,HIR,from,ancestor,Improvement,kernel,content,KOC,would,further,enhance,Using,molecular,marker,assisted,selection,combine,number,haploids,per,ear,phenotypic,criterion,developed,new,line,mean,High,can,achieve,accuracy,greater,than,different,backgrounds,reduced,false,discovery,rates,negative,previous,Comparison,strategies,suggested,that,used,during,development,could,increase,large,scale,programs,lower,cost
AB值:
0.451894
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