Soil inorganic phosphate (Pi) levels are frequently suboptimal for the growth and development of crop plants. Although MADS-box genes participate in diverse plant developmental processes, their involve-ment in phosphate starvation responses (PSRs) remains unclear. We identified a type I MADS-box tran-scription factor gene, TaMADS2-3D, which was rapidly induced under low-Pi stress in roots of wheat (Triticum aestivum). A TaMADS2-3D-GFP fusion protein was found located in the nucleus. Transgenic Arabidopsis plants overexpressing TaMADS2-3D (TaMADS2-3DOE) showed shortened primary roots, increased lateral root density, and retarded seedling growth under high-Pi (HP) conditions, accompanied by increased Pi contents in their shoots and roots. The Arabidopsis TaMADS2-3DOE plants showed similar PSR phenotypes under low Pi (LP) conditions. These results indicate constitutive activation of PSRs by overexpression of TaMADS2-3D in Arabidopsis. Reactive oxygen species (ROS), H2O2 and O2?, levels were increased in root tips of Arabidopsis TaMADS2-3DOE plants under HP conditions. Transcriptome analysis of Arabidopsis TaMADS2-3DOE plants under different Pi regimes revealed expression changes for a variety of PSR genes including AtZAT6. Overexpression of TaMADS2-3D in wheat also led to constitutive activation of PSRs. We propose that TaMADS2-3D regulates plant PSRs probably by modulating ROS homeostasis, root development, PSR gene expression, and Pi uptake. This study increases our understanding of plant PSR regulation and provides a valuable gene for improving phosphorus-use efficiency in wheat and other crops.

Yingchun Han;Na Liu;Chuang Li;Shuaiwu Wang;Lihua Jia;Rui Zhang;Hui Li;Jinfang Tan;Hongwei Xue;Wenming Zheng

Collaborative Innovation Center of Henan Grain Crops/State Key Laboratory of Wheat and Maize Crop Science,College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China;College of Resource and Environment,Henan Agricultural University,Zhengzhou 450002,Henan,China;School of Agriculture,Sun Yat-sen University,Guangzhou 510275,Guangdong,China;School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China

作物学报（英文版）

[1]Yingchun Han;Na Liu;Chuang Li;Shuaiwu Wang;Lihua Jia;Rui Zhang;Hui Li;Jinfang Tan;Hongwei Xue;Wenming Zheng-.TaMADS2-3D, a MADS transcription factor gene, regulates phosphate starvation responses in plants)[J].作物学报（英文版）,2022(01):243-253

TaMADS2,3DOE,AtZAT6

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