典型文献
Development and molecular analysis of a novel acetohydroxyacid synthase rapeseed mutant with high resistance to sulfonylurea herbicides
文献摘要：
With the increasing promotion of simplified rapeseed cultivation in recent years, the development of cul-tivars with high resistance to herbicides is urgently needed. We previously developed M342, which shows sulfonylurea herbicide resistance, by targeting acetohydroxyacid synthase (AHAS), a key enzyme in branched-chain amino acid synthesis. In the present study, we used a progeny line derived from M342 for an additional round of ethyl methane sulfonate mutagenesis, yielding the novel mutant DS3, which harbored two mutations in AHAS genes and showed high sulfonylurea resistance. One mutation was the substitution Trp574Leu, as in M342, according to Arabidopsis protein sequencing. The other site was a newly recognized substitution, Pro197Leu. A KASP marker targeting Pro197Leu was developed and reliably predicted the response to sulfonylurea herbicides in the F2 population. The combination of Trp574Leu and Pro197Leu in DS3 produced a synergistic effect that greatly increased herbicide resis-tance. Analysis of the protein structures of AHAS1 and AHAS3 in wild-type and single-gene mutant plants revealed three-dimensional protein conformational changes that could account for differences in herbi-cide resistance characteristics including toxicity tolerance, AHAS enzyme activity, and AHAS gene expression.
文献关键词：
中图分类号：
作者姓名：
Yue Guo;Changle Liu;Weihua Long;Jianqin Gao;Jiefu Zhang;Song Chen;Huiming Pu;Maolong Hu
作者机构：
Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences Key Laboratory of Cotton and Rapeseed(Nanjing),Ministry of Agriculture,Nanjing 210014,Jiangsu,China;School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,Jiangsu,China;Provincial Key Laboratory of Agrobiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China;Institute of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China
文献出处：
引用格式：
[1]Yue Guo;Changle Liu;Weihua Long;Jianqin Gao;Jiefu Zhang;Song Chen;Huiming Pu;Maolong Hu-.Development and molecular analysis of a novel acetohydroxyacid synthase rapeseed mutant with high resistance to sulfonylurea herbicides)[J].作物学报（英文版）,2022(01):56-66
A类：
acetohydroxyacid,sulfonylurea,tivars,M342,AHAS,Trp574Leu,Pro197Leu,AHAS1,AHAS3,herbi,cide
B类：
Development,molecular,analysis,novel,synthase,rapeseed,mutant,high,resistance,herbicides,With,increasing,promotion,simplified,cultivation,recent,years,development,urgently,needed,We,previously,developed,which,shows,by,targeting,key,enzyme,branched,chain,amino,synthesis,In,present,study,used,progeny,line,derived,from,additional,round,ethyl,methane,sulfonate,mutagenesis,yielding,DS3,harbored,two,mutations,showed,One,was,substitution,according,Arabidopsis,protein,sequencing,other,site,newly,recognized,KASP,marker,reliably,predicted,response,F2,population,combination,produced,synergistic,effect,that,greatly,increased,Analysis,structures,wild,type,single,plants,revealed,three,dimensional,conformational,changes,could,account,differences,characteristics,including,toxicity,tolerance,activity,expression
AB值：
0.47775
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
