典型文献
Genome assembly of the maize inbred line A188 provides a new reference genome for functional genomics
文献摘要：
The current assembled maize genomes cannot represent the broad genetic diversity of maize germ-plasms. Acquiring more genome sequences is critical for constructing a pan-genome and elucidating the linkage between genotype and phenotype in maize. Here we describe the genome sequence and annotation of A188, a maize inbred line with high phenotypic variation relative to other lines, acquired by single-molecule sequencing and optical genome mapping. We assembled a 2210-Mb genome with a scaffold N50 size of 11.61 million bases (Mb), compared to 9.73 Mb for B73 and 10.2 Mb for Mo17. Based on the B73_RefGen_V4 genome, 295 scaffolds (2084.35 Mb, 94.30％of the final genome assembly) were anchored and oriented on ten chromosomes. Comparative analysis revealed that ～30％ of the predicted A188 genes showed large structural divergence from B73, Mo17, and W22 genomes, which causes high protein divergence and may lead to phenotypic variation among the four inbred lines. As a line with high embryonic callus (EC) induction capacity, A188 provides a convenient tool for elucidating the molecular mechanism underlying the formation of EC in maize. Combining our new A188 genome with previously reported QTL and RNA sequencing data revealed eight genes with large structural variation and two dif-ferentially expressed genes playing potential roles in maize EC induction.
文献关键词：
中图分类号：
作者姓名：
Fei Ge;Jingtao Qu;Peng Liu;Lang Pan;Chaoying Zou;Guangsheng Yuan;Cong Yang;Guangtang Pan;Jianwei Huang;Langlang Ma;Yaou Shen
作者机构：
Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region,Maize Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Berry Genomics Corporation,Beijing 100015,China
文献出处：
引用格式：
[1]Fei Ge;Jingtao Qu;Peng Liu;Lang Pan;Chaoying Zou;Guangsheng Yuan;Cong Yang;Guangtang Pan;Jianwei Huang;Langlang Ma;Yaou Shen-.Genome assembly of the maize inbred line A188 provides a new reference genome for functional genomics)[J].作物学报（英文版）,2022(01):47-55
A类：
RefGen
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AB值：
0.555777
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