Species of the Pinus genus provide a classical model for studying hybrid speciation.Although studies on two narrowly distributed species(Pinus funebris and P.taka-hasii)concluded that they originated from two widespread species(P.sylvestris and P.densiflora)via hybrid speciation,the conclusion was based on a low number of informative restriction sites.In this study,we analyzed the sequences of four Pinus chloroplast(cp)genomes(P.sylvestris,P.densi-flora,P.funebris and P.takahasii)to clarify whether hybrid speciation was involved.The complete cp-genomes of Pinus species ranged in size from 119,865 to 119,890 bp,similar to other Pinus species.Phylogenetic results based on the whole cp-genomes showed P.sylvestris clustered with P.funebris and P.takahasii,which suggested that P.sylves-tris was the paternal parent in hybridization events.In an analysis of simple sequence repeats(SSRs),we detected a total of 69 SSRs repeats among the four Pinus cp-genomes;most were A or T bases.In addition,we identified divergent hotspot regions among the four Pinus cp-genomes(trnE-clpP,cemA-ycf4,petD-rpoA,psbD-trnT,and trnN-chlL),in P.sylvestris(psbD-trnT,trnN-chlL,psbB and rps8)and in P.densiflora(trnE-clpP,petD-rpoA,ycf3 intron,psbD-trnT,and trnN-chlL).The genome information found in this study provides new insights into hybrid speciation in Pinus and contributes to a better understanding of the phylogenetic relationships within the Pinus genus.

Tao Yu;Zhiyuan Jia;Buddhi Dayananda;Junqing Li;Xiaolei Guo;Liang Shi;Xiaowen Yuan;Yan Gao

Beijing Key Laboratory for Forest Resources and Ecosystem Processes,Beijing Forestry University,Beijing 100083,People's Republic of China;School of Agriculture and Food Sciences,The University of Queensland,Brisbane,QLD 4072,Australia;Kunyu Mountain National Nature Reserve Administration,Yantai 264113,People's Republic of China;Yantai Kunyu Mountain Forest Farm,Yantai 264113,People's Republic of China

林业研究（英文版）

[1]Tao Yu;Zhiyuan Jia;Buddhi Dayananda;Junqing Li;Xiaolei Guo;Liang Shi;Xiaowen Yuan;Yan Gao-.Analysis of the chloroplast genomes of four Pinus species in Northeast China:Insights into hybrid speciation and identification of DNA molecular markers)[J].林业研究（英文版）,2022(06):1881-1890

taka,hasii,takahasii,sylves,chlL,psbB,rps8

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