首站-论文投稿智能助手
首页
期刊导航
职称导航
中图分类号
典型文献
Tomato mottle mosaic virus: Characterization, resistance gene effectiveness, and quintuplex RT-PCR detection system
文献摘要：
Tomato mottle mosaic virus (ToMMV), an economically important species of the genus Tobamovirus, causes significant loss in yield and quality of tomato fruits. Here, we identified the Shandong isolate of ToMMV (ToMMV-SD) collected from symptomatic tomato fruits in Weifang, Shandong Province of China. ToMMV-SD caused symptoms such as severe mosaic, mottling, and necrosis of tomato leaves, yellow spot and necrotic lesions on tomato fruits. The obtained full genome of ToMMV-SD was 6399 nucleotides (accession number MW373515) and had the highest identity of 99.5％ with that of isolate SC13-051 from the United States of America at the genomic level. The infectious clone of ToMMV-SD was constructed and induced clear mosaic and necrotic symptoms onto Nicotiana benthamiana leaves. Several commercial tomato cultivars, harboring Tm-22 resistance gene, and pepper cultivars, containing L resistance gene, were susceptible to ToMMV-SD. Plants of Solanum melongena (eggplant) and Brassica pekinensis (napa cabbage) showed mottling symptoms, while N. tabacum cv. Zhongyan 100 displayed latent infection. ToMMV-SD did not infect plants of N. tabacum cv. Xanthi NN, Brassica rapa ssp. chinensis (bok choy), Raphanus sativus (radish), Vigna unguiculata cv. Yuanzhong 28-2 (cowpea), or Tm-22 transgenic N. benthamiana. A quintuplex RT-PCR system differentiated ToMMV from tomato mosaic virus, tomato brown rugose fruit virus, tobacco mosaic virus, and tomato spotted wilt virus, with the threshold amount of 0.02 pg. These results highlight the threat posed by ToMMV to tomato and pepper cultivation and offer an efficient detection system for the simultaneous detection of four tobamoviruses and tomato spotted wilt virus infecting tomato plants in the field.
文献关键词：
中图分类号：
[1] 医药、卫生（R） / 中国医学（R2） / 中药学（R28） / 中药药理学（R285） / 中药实验药理（R285.5）
[2] 医药、卫生（R） / 药学（R9） / 药理学（R96） / 实验药理学（R965）
[3] 农业科学（S） / 畜牧、动物医学、狩猎、蚕、蜂（S8） / 动物医学（兽医学）（S85） / 兽医基础科学（S852） / 家畜微生物学（兽医病原微生物学）（S852.6） / 家畜病毒学（S852.65）
作者姓名：
Carlos Kwesi TETTEY;YAN Zhi-yong;MA Hua-yu;ZHAO Mei-sheng;GENG Chao;TIAN Yan-ping;LI Xiang-dong
作者机构：
Shandong Provincial Key Laboratory of Agricultural Microbiology,College of Plant Protection,Shandong Agricultural University,Tai'an 271018,P.R.China
文献出处：
农业科学学报（英文）
引用格式：
[1]Carlos Kwesi TETTEY;YAN Zhi-yong;MA Hua-yu;ZHAO Mei-sheng;GENG Chao;TIAN Yan-ping;LI Xiang-dong-.Tomato mottle mosaic virus: Characterization, resistance gene effectiveness, and quintuplex RT-PCR detection system)[J].农业科学学报（英文）,2022(09):2641-2651
A类：
quintuplex,ToMMV,Tobamovirus,Weifang,mottling,MW373515,SC13,napa,Zhongyan,Xanthi,bok,choy,Yuanzhong,tobamoviruses
B类：
Tomato,mottle,mosaic,Characterization,resistance,gene,effectiveness,detection,system,economically,important,species,genus,causes,significant,loss,yield,quality,tomato,fruits,Here,identified,Shandong,isolate,collected,from,symptomatic,Province,China,caused,symptoms,such,severe,necrosis,leaves,yellow,necrotic,lesions,obtained,full,genome,was,nucleotides,accession,number,had,highest,identity,that,United,States,America,genomic,level,infectious,clone,constructed,induced,clear,onto,Nicotiana,benthamiana,Several,commercial,cultivars,harboring,Tm,pepper,containing,were,susceptible,Plants,Solanum,melongena,eggplant,Brassica,pekinensis,cabbage,showed,while,tabacum,cv,displayed,latent,infection,did,not,plants,NN,rapa,ssp,chinensis,Raphanus,sativus,radish,Vigna,unguiculata,cowpea,transgenic,differentiated,brown,rugose,tobacco,spotted,wilt,threshold,amount,pg,These,results,highlight,threat,posed,by,cultivation,offer,efficient,simultaneous,four,infecting,field
AB值：
0.48216
相似文献
Fine mapping of an adult-plant resistance gene to powdery mildew in soybean cultivar Zhonghuang 24
Qian Zhou;Bingzhi Jiang;Yanbo Cheng;Qibin Ma;Qiuju Xia;Ze Jiang;Zhandong Cai;Hai Nian-The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,Guangdong,China;The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology,South China Agricultural University,Guangzhou 510642,Guangdong,China;The Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Guangdong Provincial Key Laboratory of Crops Genetics and Improvement,Crops Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510642,Guangdong,China;Beijing Genomics Institute(BGI)Education Center,University of Chinese Academy of Sciences,Shenzhen 518083,Guangdong,China
The jasmonate-induced bHLH gene SlJIG functions in terpene biosynthesis and resistance to insects and fungus
Yunyun Cao;Lun Liu;Kangsheng Ma;Wenjing Wang;Hongmei Lv;Ming Gao;Xinman Wang;Xichun Zhang;Shuxin Ren;Na Zhang;Yang-Dong Guo-College of Horticulture,China Agricultural University,Beijing 100193,China;College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an 271018,China;Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;School of Agriculture,Virginia State University,Petersburg 23806,VA,USA;Sanya Institute of China Agricultural University,Sanya 572000,China
The lncRNA39896–miR166b–HDZs module affects tomato resistance to Phytophthora infestans
Yuhui Hong;Yuanyuan Zhang;Jun Cui;Jun Meng;Yinhua Chen;Chengwei Zhang;Jinxiao Yang;Yushi Luan-School of Bioengineering,Dalian University of Technology,Dalian 116024,China;State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 311400,China;College of Life Science,Hunan Normal University,Changsha 410081,China;School of Computer Science and Technology,Dalian University of Technology,Dalian 116024,China;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource,College of Tropical Crops,Hainan University,Haikou 570228,China;Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding,Beijing Academy of Agriculture&Forestry Sciences,Beijing 100000,China
BioClay?prolongs RNA interference-mediated crop protection against Botrytis cinerea
Jonatan Ni?o-Sánchez;Prabhakaran T.Sambasivam;Anne Sawyer;Rachael Hamby;Angela Chen;Elizabeth Czislowski;Peng Li;Narelle Manzie;Donald M.Gardiner;Rebecca Ford;Zhi Ping Xu;Neena Mitter;Hailing Jin-Department of Microbiology&Plant Pathology,Center for Plant Cell Biology,Institute for Integrative Genome Biology,University of California,RiversideCalifornia,92521,USA;Department of Plant Production and Forest Resources,University of Valladolid,Palencia 34004,Spain;iuFOR-Sustainable Forest Management Research Institute,University of Valladolid–INIA,Palencia 34004,Spain;Centre for Planetary Health and Food Security,Griffith University,Nathan,Queensland,4111,Australia;School of Chemistry and Molecular Biosciences,The University of Queensland,St Lucia,Queensland,4072,Australia;Queensland Alliance for Agriculture and Food Innovation,Centre for Horticultural Science,The University of Queensland,St Lucia,Queensland,4072,Australia;School of Agriculture and Food Sciences,The University of Queensland,St Lucia,Queensland,4072,Australia;Australian Institute for Bioengineering and Nanotechnology,The University of Queensland,St Lucia,Queensland,4072,Australia
Rootstock-scion interactions affect fruit flavor in grafted tomato
Zhen Zhou;Yaqin Yuan;Ketao Wang;Haijing Wang;Jianqin Huang;Hong Yu;Xia Cui-Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Sino-Dutch Joint Laboratory of Horticultural Genomics,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;China State Key Laboratory of Subtropical Forest Cultivation,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China;State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China
Two plant NLR proteins confer strain-specific resistance conditioned by an effector from Pseudomonas syringae pv.actinidiae
Xiaojuan Zheng;Zhaoyang Zhou;Zhen Gong;Meijuan Hu;Ye Jin Ahn;Xiaojuan Zhang;Yan Zhao;Guoshu Gong;Jian Zhang;Jianru Zuo;Guan-Zhu Han;Sohn Kee Hoon;Jian-Min Zhou-State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China;CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China;College of Horticulture,China Agricultural University,Beijing 100193,China;College of Life Sciences,Jiangsu Key Laboratory for Microbes and Functional Genomics,Nanjing Normal University,Nanjing,Jiangsu 210023,China;Department of Life Sciences,Pohang University of Science and Technology,Pohang 37673,Republic of Korea;School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology,Pohang 37673,Republic of Korea;Plant Protection Department and Major Crop Disease Laboratory,College of Agronomy,Sichuan Agricultural University,Chengdu,Sichuan 611130,China;Hainan Yazhou Bay Seed Laboratory,Sanya,Hainan 572025,China
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
客服邮箱：957655803@qq.com
渝公网安备：50011902000454号
ICP备案号：渝ICP备2023002303号
重庆域田数据科技有限公司©版权所有
官方客服