Mechanisms controlling phosphorus (P) availability and the roles of microorganisms in the efficient utilization of soil P in the wheat–maize double cropping system are poorly understood. In the present study, we conducted a pot experiment for four consecutive wheat–maize seasons (2016–2018) using calcareous soils with high (30.36 mg kg–1) and low (9.78 mg kg–1) initial Olsen-P content to evaluate the effects of conventional P fertilizer application to both wheat and maize (Pwm) along with a reduced P fertilizer application only to wheat (Pw). The microbial community structure along with soil P availability parameters and crop yield were determined. The results showed that the Pw treatment reduces the annual P input by 33.3％ without affecting the total yield for at least two consecutive years as compared with the Pwm treatment in the high Olsen-P soil. Soil water-soluble P concentrations in the Pw treatment were similar to those in the Pwm treatment at the 12-leaf collar stage when maize requires the most P. Furthermore, the soil P content significantly affected soil microbial communities, especially fungal communities. Meanwhile, the relative abundances of Proteobacteria and alkaline phosphatase (ALP) activity of Pw were significantly higher (by 11.4 and 13.3％) than those of Pwm in soil with high Olsen-P. The microfloral contribution to yield was greater than that of soil P content in soil with high Olsen-P. Relative abundances of Bacillus and Rhizobium were enriched in the Pw treatment compared with the Pwm treatment. Bacillus showed a significant positive correlation with acid phosphatase (ACP) activity, and Rhizobium displayed significant positive correlations with ACP and ALP in soil with high Olsen-P, which may enhance P availability. Our findings suggested that the application of P fertilization only to wheat is practical in high P soils to ensure optimal production in the wheat and maize double cropping system and that the soil P availability and microbial community may collaborate to maintain optimal yield in a wheat–maize double cropping system.

YU Xiao-jing;CHEN Qi;SHI Wen-cong;GAO Zheng;SUN Xiao;DONG Jing-jing;LI Juan;WANG Heng-tao;GAO Jian-guo;LIU Zhi-guang;ZHANG Min

National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources/College of Resources and Environment/College of Life Sciences,Shandong Agricultural University,Tai'an 271018,P.R.China;Key Laboratory of Plant Nutrition and Fertilizer,Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;Shandong Zhongxin Agricultural Development Co.,Ltd.,Linshu 276700,P.R.China

农业科学学报（英文）

[1]YU Xiao-jing;CHEN Qi;SHI Wen-cong;GAO Zheng;SUN Xiao;DONG Jing-jing;LI Juan;WANG Heng-tao;GAO Jian-guo;LIU Zhi-guang;ZHANG Min-.Interactions between phosphorus availability and microbes in a wheat–maize double cropping system: A reduced fertilization scheme)[J].农业科学学报（英文）,2022(03):840-854

Pwm,Pw,microfloral

Interactions,between,phosphorus,availability,microbes,wheat,maize,double,cropping,system,reduced,fertilization,scheme,Mechanisms,controlling,roles,microorganisms,efficient,utilization,poorly,understood,present,study,conducted,pot,experiment,four,consecutive,seasons,using,calcareous,soils,low,initial,Olsen,content,evaluate,effects,conventional,fertilizer,application,both,along,only,microbial,community,structure,parameters,yield,were,determined,results,showed,that,treatment,reduces,annual,input,by,without,affecting,total,least,two,years,compared,Soil,water,soluble,concentrations,similar,those,leaf,collar,stage,when,requires,most,Furthermore,significantly,affected,communities,especially,fungal,Meanwhile,relative,abundances,Proteobacteria,alkaline,phosphatase,ALP,activity,higher,than,contribution,was,greater,Relative,Bacillus,Rhizobium,enriched,positive,acid,ACP,displayed,correlations,which,may,enhance,Our,findings,suggested,practical,ensure,optimal,production,collaborate,maintain

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